A combined approach utilizing UAV 3D imaging methods, in-situ measurements, and laboratory experiments to assess water evaporation and trace element uptake by tree species growing in a red gypsum landfill.

The extractive industry is increasingly faced with problems of managing contaminated sites. The red gypsum landfill at the Ochsenfeld site is representative of the typology byproduct storage of the Ti-extraction activity. The management of the elemental content and the water body are the issues at this site. The aim of this study was to evaluate the canopy conductance (gcmax) of various tree species and the content of elements in the leaves, utilizing the opportunity of a demonstration plantation setup in 2014 with sixteen tree species, combined with a growth chamber experiment. We combined the gas exchange measurements with the data from two multispectral cameras with RGB and NIR bands embarked on an unmanned aerial vehicle (UAV). In the field, Ostrya carpinifolia, Maclura pomifera, and Rhus copallina had the highest gcmax of all planted tree species, and the high transpiration rate in O. carpinifolia was confirmed in a pot-based controlled experiment. Except R. copallina, the species with a high Mn content (O. carpinifolia, Betula pendula, and Salix aquatica grandis) had high stomatal conductance. O. carpinifolia could therefore be a species to exploit in the management of landfill leachates, especially in the context of climate change since this species is well adapted to dry environments.

Trace Metal(oid) Accumulation in Edible Crops and Poplar Cuttings Grown on Dredged Sediment Enriched Soil.

The development of a biomonitor in the context of multiple-element contamination in urban environments was tested by comparing element transfer in edible crops and poplar ( Torr. × A. Henry cutlivar 'Skado'). A multielemental analysis was performed with various common edible crops (cucumber [ L.], pepper [ L.], cabbage [ L.], and lettuce [ L.]) and the Skado poplar cultivar grown on soils that received sediments dredged from water canals in the 1960s. Sediments were distributed unevenly on the soil, allowing us to sample two types of areas that were either weakly (Area 1) or highly (Area 2) contaminated, mainly by Cd, Pb, and Zn. We registered an accumulation of Cd and Zn in the edible parts of crops, with higher values recorded for leafy vegetables than for fruit vegetables. We did not detect any accumulation of Pb in the plant species studied. We calculated the fresh mass that must be consumed daily to reach tolerable daily intake (TDI) recommendations for each element and found evidence that Cd could be ingested in sufficient amounts to reach the TDI in this context. Poplar and pepper leaves accumulated more Cd and Zn than the edible parts of the study crops grown on both substrates, which suggests that poplar and pepper may be suitable species for biomonitoring element transfer to vegetation in this context.

Accumulation of trace elements in edible crops and poplar grown on a titanium ore landfill.

Urban gardening has recently experienced rapid development; however, the risk of the transfer of toxic elements from neighboring industry needs to be evaluated. We performed a multi-elemental analysis with several common edible crops (cucumber, pepper, cabbage, and lettuce) and poplar grown directly on a titanium ore landfill as a maximized scenario of exposure. Despite elevated concentrations of soil Ca, Fe, Mn, and Ti resulting from the industrial process, we did not register higher accumulation of these elements in the edible parts of crops or in poplar leaves grown on red gypsum compared with the control soil. Only S concentrations were higher in plants grown on the red gypsum, especially for cabbage. The principal component analysis among elements for plants grown on red gypsum indicated that S and Mn were accumulated by different plant species than Cd, Cu, and Zn. The poplar clone had a significantly higher transfer of S and Cr than the control and is a suitable tree species for monitoring element transfer to vegetation in this industrial context. By comparing our data with tolerable daily intake (TDI) recommendations, we demonstrated the low risk of cultivating edible crops directly on an industrial substrate in a maximized scenario of exposure, except for Cr, for which the toxicity depends on the bioavailable form. However, we did not consider the cumulative effects of the various elements because there are no current guidelines, and further research is needed to address this question.

Mercury uptake into poplar leaves.

Tailings dumps require mercury stabilization to prevent air pollution by evaporated mercury, which can be achieved through plant covers. Plants are considered a net sink for atmospheric Hg via incorporation into leaf tissues. However, most studies related to Hg uptake by plants have considered plants exposed to only atmospheric Hg, whereas in the case of tailings dumps, plants are potentially exposed to both soil and atmospheric Hg. The goal of this work is to evaluate the relative contributions of root and atmospheric pathways by growing poplar (Populus trichocarpa X Populus maximowiczii/var Skado) cuttings on either control or polluted substrates and under either natural or controlled exposure conditions. We showed that foliar Hg concentrations significantly increased with age, reaching 120 ng g(-1) dry mass when poplars were exposed to Hg-contaminated substrate under natural exposure. Remarkably, we did not observe significantly different Hg concentrations in poplar leaves grown on either the control or polluted substrates when cultivated together in growth chambers. Our set of data prompted us to conclude that Hg entry into poplar leaves is exclusively through an atmospheric pathway. Our results are discussed in line with existing literature.

Short-term response to waterlogging in Quercus petraea and Quercus robur: A study of the root hydraulic responses and the transcriptional pattern of aquaporins.

We characterized the short-term response to waterlogging in Quercus petraea (Matt.) Liebl. and Quercus robur L. as the initial response towards their known long-term differences in tolerance to waterlogging. One-month old seedlings were subjected to hypoxic stress and leaf gas exchange, shoot water potential (Ψs) and root hydraulic conductivity (Lpr) were measured. In parallel, the expression of nine aquaporins (AQPs) along the primary root was analysed by quantitative RT-PCR. Results showed a similar reduction in net assimilation (A) and stomatal conductance (gs) for the two species. Notably, the response of Lpr differed temporally between the two species. Q. robur seedlings exhibited a significant early decline of Lpr within the first 5 h that returned to control levels after 48 h, whereas Q. petraea seedlings showed a delayed response with a significant decrease of Lpr exhibited only after 48 h. Transcriptional profiling revealed that three genes (PIP1;3, TIP2;1 and TIP2;2) were differentially regulated under stress conditions in the two oak species. Taken together, these results suggested species-specific responses to short-term waterlogging in terms of root water transport.

Identification and expression of nine oak aquaporin genes in the primary root axis of two oak species, Quercus petraea and Quercus robur.

Aquaporins (AQPs) belong to the Major Intrinsic Protein family that conducts water and other small solutes across biological membranes. This study aimed to identify and characterize AQP genes in the primary root axis of two oak species, Quercus petraea and Quercus robur. Nine putative AQP genes were cloned, and their expression was profiled in different developmental root zones by real-time PCR. A detailed examination of the predicted amino acid sequences and subsequent phylogenetic analysis showed that the isolated AQPs could be divided into two subfamilies, which included six plasma membrane intrinsic proteins (PIPs) and three tonoplast intrinsic proteins (TIPs). We characterized the anatomical features of the roots and defined three developmental root zones: the immature, transition and mature zones. Expression analysis of the AQPs was performed according to these root developmental stages. Our results showed that the expression of PIP2;3 and TIP1 was significantly higher in Quercus petraea compared with Quercus robur in the three root zones. However, PIP2;1 and TIP2;1 were found to be differentially expressed in the mature zone of the two oak species. Of the nine AQP genes identified and analyzed, we highlighted four genes that might facilitate a deeper understanding of how these two closely related tree species adapted to different environments.